Dendritic cell assays
Isolated monocytes from fresh healthy donor whole blood were differentiated into dendritic cells, validated by cytokine profiling and surface marker expression. Suitable in vitro assay models for measuring a compound’s effect on dendritic cell function and gene expression.
Dendritic cell differentiation
Figure-A: Methods for isolation of monocytes, maintenance, and differentiation into dendritic cells.
Cytokine levels
Figure-B: IL-12 (p70) levels (pg/ml) in dendritic cell supernatant.
Surface marker expression levels
Figure-C: Surface marker expression levels in mature dendritic cells measured by flowcytometry.
Here we demonstrate the successful differentiation of monocytes into dendritic cells. Our in vitro assay models enabled us to support client’s therapeutic programmes, measuring the compound’s effect on dendritic cell maturation, function, and gene expression.