Expression & Functional studies

Expression Studies: Expression studies with normal and diseased models may provide better understanding of associated pathways and disease biology. Compound profiling for drug effects on target expression can help to gain insights into drug interactions with targets, therapeutics efficacy and any associated drug toxicity. Expression studies include:

  • Target expression levels and compound profiling
  • Assay formats include: Western blotting (WB), qPCR, ELISA, HTRF and AlphaLisa assays
  • Transfections (transient/ stable) and transductions
  • Transcription factor-activation profiling specific to project (e.g., cancer,  neurodegenerative, T-cell activation, insulin resistance, etc.)
  • Sample type: cells, blood and tissues (e.g., from xenograft studies)

Functional Assays: Functional assays assist in understanding the ability of drugs to regulate signalling pathways and intracellular messengers. Regulation of a specific signalling pathway may have therapeutic merit and functional assays could help improve the therapeutic efficacy of a drug and reduce adverse effects. Functional assays include: cAMP assays, IP-1 assays, b-Arrestin assays, Ca flux assays, Phosphorylation levels, Gene reporter assays and Inflammation & GPCR signalling pathways.