GPCRs are popular and well-established protein targets, representing the largest individual family of drug targets used in current medication. BioMedha offers custom made GPCR assay development and screening services to suit your needs for interrogating compounds against your target of interest.

Our custom GPCR assay services include:

  • Constitutive activity check, transient & stable transfections
  • Stable cell line generation, assay development, and assay optimisation
  • Single receptor, dual, and receptor dimerisation
  • Supporting screening cascade: species and target selectivity assays
  • Biased signaling, allosteric and orthosteric modulation
  • MOA studies
  • Shield analysis

GPCR functional assay formats include:

  • β-Arrestin recruitment
  • cAMP levels (Gi and Gs)
  • IP-1 levels and Ca flux (Gq)
  • Phospho-proteins (e.g. pERK1/2 levels)
  • nanoBRET in cell target engagement
  • Receptor internalization

To learn more about how we can support your projects, get in touch with our team.